Preparation of Membrane Fractions With Enhanced Tumor-Transplantation-Antigen Activity From Tumor Cells Infected With Influenza Virus
- 1 October 1973
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 51 (4) , 1141-1144
- https://doi.org/10.1093/jnci/51.4.1141
Abstract
Whereas the cell membrane fraction of a transplantable simian virus 40 (SV40)-transformed fibrosarcoma of BALB/c mice was without tumor transplantation antigen (TTA) activity, the same fraction from cells infected in vitro with influenza virus retained TTA activity to a degree approaching that of whole cells. This enhancement of immunogenicity was specific: Immunization of mice with homogenates of influenza virus-infected normal cells or spontaneously transformed tumor cells did not confer immunity to challenge with the SV40-tumor cells. The virus-enhanced TTA activity was associated with cell membrane fractions isolated either on sucrose density gradients or from phosphate-buffered saline eluates of frozen and thawed tumor cells.Keywords
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