Proton-NMR assignment and secondary structural elements of human transforming growth factor
- 24 January 1989
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 28 (2) , 593-599
- https://doi.org/10.1021/bi00428a027
Abstract
The 1H NMR spectrum of human transforming growth factor .alpha. (hTGF-.alpha.) has been completely assigned, and secondary structural elements have been identified as a preliminary step in determining the structure of this protein by distance geometry methods. Many of these structural elements closely correspond to those previously found in a truncated human EGF [Cooke et al. (1987) Nature (London) 327, 339-341] and murine EGF [Montelione et al. (1987) Proc. Natl. Acad. Sci. U.S.A. 84, 5226-5230]. These include the presence of an antiparallel .beta.-sheet between residues G19 and C34 with a type I .beta.-turn at V25-D28, a type II .beta.-turn at H35-Y38, and another short .beta.-sheet between residues Y38-V39 and H45-A46.This publication has 18 references indexed in Scilit:
- Rat epidermal growth factor: complete amino acid sequenceEuropean Journal of Biochemistry, 1985
- Vaccinia virus-infected cells release a novel polypeptide functionally related to transforming and epidermal growth factors.Proceedings of the National Academy of Sciences, 1985
- Polypeptide secondary structure determination by nuclear magnetic resonance observation of short proton-proton distancesJournal of Molecular Biology, 1984
- Two‐dimensional 1H NMR of two chemically modified analogs of the basic pancreatic trypsin inhibitorEuropean Journal of Biochemistry, 1984
- Human transforming growth factor-α: Precursor structure and expression in E. coliPublished by Elsevier ,1984
- Improved spectral resolution in COSY 1H NMR spectra of proteins via double quantum filteringBiochemical and Biophysical Research Communications, 1983
- Epidermal growth factor-like transforming growth factor. II. Interaction with epidermal growth factor receptors in human placenta membranes and A431 cells.Journal of Biological Chemistry, 1983
- Sequential resonance assignments in protein 1H nuclear magnetic resonance spectraJournal of Molecular Biology, 1982
- Transforming growth factors produced by certain human tumor cells: polypeptides that interact with epidermal growth factor receptors.Proceedings of the National Academy of Sciences, 1980
- β-turns in proteinsJournal of Molecular Biology, 1977