Resolution of bovine brain calcineurin subunits: stimulatory effect of subunit B on subunit A phosphatase activity
- 7 October 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 190 (1) , 104-108
- https://doi.org/10.1016/0014-5793(85)80437-3
Abstract
Calcineurin was dissociated into subunits A and B by SDS and the dissociated subunits were separated by Sephadex G‐100 column chromatography in SDS. The phosphatase activity was associated with the A subunit and was detected only in the presence of MnCl2 of the various divalent cations tested. The Mn2+‐dependent phosphatase of A subunit was stimulated (4‐5‐fold) by calmodulin. The subunit B increased only modestly Mn2+ stimulated phosphatase activity of subunit A but markedly increased it when assay also contained calmodulin. These results support the view that subunit B plays an important role in Mn2+/calmodulin regulation of subunit A phosphatase activity. They also lend further support to our earlier postulate ([1984] FEBS Lett. 169, 251‐255) that Mn2+ is a powerful regulator of calcineurin phosphatase.Keywords
This publication has 11 references indexed in Scilit:
- A multifunctional calmodulin-stimulated phosphataseArchives of Biochemistry and Biophysics, 1985
- Catalytic site of calmodulin-dependent protein phosphatase from bovine brain resides in subunit A.Proceedings of the National Academy of Sciences, 1984
- Intrinsic phosphatase activity of bovine brain calcineurin requires a tightly bound trace metalFEBS Letters, 1984
- The structure of the B subunit of calcineurinEuropean Journal of Biochemistry, 1984
- Activation of calcineurin by limited proteolysis.Proceedings of the National Academy of Sciences, 1983
- Calmodulin interacts with cyclic nucleotide phosphodiesterase and calcineurin by binding to a metal ion-independent hydrophobic region on these proteins.Journal of Biological Chemistry, 1983
- Calcineurin is a calmodulin-dependent protein phosphataseBiochemical and Biophysical Research Communications, 1982
- Discovery of A Ca2+‐and calmodulin‐dependent protein phosphataseFEBS Letters, 1982
- Calcineurin: a calcium- and calmodulin-binding protein of the nervous system.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970