Identification of enolase as a plasminogen‐binding protein in excretory–secretory products of Fasciola hepatica
- 23 March 2004
- journal article
- Published by Wiley in FEBS Letters
- Vol. 563 (1-3) , 203-206
- https://doi.org/10.1016/s0014-5793(04)00306-0
Abstract
We have followed a combined proteomic approach to identify proteins of Fasciola hepatica that could be involved in host-parasite interactions. Using two-dimensional gel electrophoresis, far Western immunoblot and mass spectrometry analyses, we have identified the enolase enzyme, present in the excretory/secretory materials of F. hepatica, as a human plasminogen-binding protein. This enzyme has an apparent molecular weight of 47 kDa with pI ranging from 6.2 to 7.2. These results suggest that enolase could act as a plasminogen receptor.Keywords
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