Automated Fluorometric Micromethod for Blood Glucose
Open Access
- 1 November 1973
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 19 (11) , 1296-1299
- https://doi.org/10.1093/clinchem/19.11.1296
Abstract
We describe a sensitive automated fluorometric method for glucose in whole blood spotted on filter paper. By action of glucose oxidase (EC 1.1.3.4) on glucose, hydrogen peroxide is produced, which then reacts with 4-hydroxy-3-methoxy-phenylacetic acid (homovanillic acid) in the presence of horseradish peroxidase (EC 1.11.1.7) to form a fluorescent condensation product. Obtained at a rate of 60 samples per hour, the results are accurate, linear, and reproducible. Whole blood glucose can be determined by this method with an error (CV) of less than 5%.Keywords
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