Measurement of Cellulolytic Activity by Low Pressure Liquid Chromatography
- 1 January 1979
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 2 (5) , 745-760
- https://doi.org/10.1080/01483917908060100
Abstract
The application of aqueous, low pressure liquid chromatography to the assay of cellulolytic enzyme activity is discussed. The advantages of this method are speed of analysis (less than 20 min), small sample size (20 .mu.l), good resolution, and a tolerance of the system to the presence of extraneous salts and proteins. Examples showing the use of this tool are given. [Cellulase from Trichoderma reesei were used.].This publication has 14 references indexed in Scilit:
- Enzymic activities of endo-1,4-β-d-glucanases purified from Trichoderma virideBiochimica et Biophysica Acta (BBA) - Enzymology, 1978
- High-speed liquid chromatography of cellodextrins and other saccharide mixtures using water as the eluentJournal of Chromatography A, 1978
- Complexes of carbohydrates with aluminate ion. Chromatography of carbohydrates on columns of anion-exchange resin (aluminate form)Carbohydrate Research, 1975
- Separation of oligosaccharides by partition chromatography on ion exchange resinsAnalytical Chemistry, 1975
- Reducing value methods for maltodextrinsAnalytical Biochemistry, 1972
- Reducing value methods for maltodextrinsAnalytical Biochemistry, 1972
- Partition chromatography of sugars on ion-exchange resinsJournal of Chromatography A, 1970
- Colorimetric Method for Determination of Sugars and Related SubstancesAnalytical Chemistry, 1956
- A PHOTOMETRIC ADAPTATION OF THE SOMOGYI METHOD FOR THE DETERMINATION OF GLUCOSEJournal of Biological Chemistry, 1944