Genome-scale loss-of-function screening with a lentiviral RNAi library
- 23 August 2006
- journal article
- review article
- Published by Springer Nature in Nature Methods
- Vol. 3 (9) , 715-719
- https://doi.org/10.1038/nmeth924
Abstract
The discovery that RNA interference (RNAi) is functional in mammalian cells led us to form The RNAi Consortium (TRC) with the goal of enabling large-scale loss-of-function screens through the development of genome-scale RNAi libraries and methodologies for their use. These resources form the basis of a loss-of-function screening platform created at the Broad Institute. Our human and mouse libraries currently contain >135,000 lentiviral clones targeting 27,000 genes. Initial screening efforts have demonstrated that these libraries and methods are practical and powerful tools for high-throughput lentivirus RNAi screens.Keywords
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