An Embedding Method for Histochemical Studies of Undecalcified Skeletal Growth Plate
- 1 January 1980
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 55 (1) , 19-29
- https://doi.org/10.3109/10520298009067891
Abstract
We have used glycol methacrylate to study undecalcified skeletal growth plate and subchondral bone. Minor modifications of the original technique including dehydration in glycol methacrylate vacuum infiltration and polymerization in the cold make it quite suitable for embedding of such tissues. Moreover, specimens can be processed quickly and the morphologic and biochemical integrity of the tissue retained so that histochemical procedures can be readily applied. Collagen, glycosaminoglycan, glycogen, lipid, calcium and the activity of alkaline and acid phosphatase were localized. This technique appears to be very useful for studying skeletal tissues.This publication has 34 references indexed in Scilit:
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