Isolation and Characterization of a Filamentous Phage, Vf33, Specific for Vibrio parahaemolyticus

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Abstract
Phage Vf33, a filamentous phage .apprx. 1400 nm long and 7 nm wide, specific for V. parahaemolyticus, were isolated and characterized. The buoyant density of Vf33 in CsCl was 1.292 g/cm3. As with other filamentous phages, the lytic activity of Vf33 was resistant to heating below 80.degree. C and to treatment with diethylether, acetone or methanol but sensitive to CHCl3. The nucleic acid of this phage is single-stranded circular DNA 8.4 kilobases in size. The viral genome was converted to a double-stranded replicative form in the host cell. Among the strains tested, only V. parahaemolyticus strains possessing K38 antigen was sensitive to the phage.