Enzyme cytochemical study of rat cardiac muscle II. Ca++-ATPase and ouabain-sensitive, K+-dependent p-nitrophenylphosphatase.

1 January 1982

journal article
research article
Published by Japan Society of Histochemistry & Cytochemistry in ACTA HISTOCHEMICA ET CYTOCHEMICA

Vol. 15 (3) , 338-354
https://doi.org/10.1267/ahc.15.338

Abstract

Myocardial Ca2+-activatedATPase (Ca2+-ATPase) and ouabain-sensitive, K+-dependent p-nitrophenylphosphatase (K+-NPPase) were demonstrated by the method of Ando (1981) and Mayahara (1980). At pH 9.0, Ca2+-ATPase activity was localized on the myofilaments, sarcoplasmic reticulum, gap junction and the matrix of mitochondria. Sarcolemmal ATPase activity could be detected at neutral pH. K+-NPPase was seen on the cytoplasmic side of the sarcolemma and T-tubule. These findings support the role of these structures for intracellular Ca2+-Na+ (probably also K+ also related) interaction inducing the heart activity.

This publication has 1 reference indexed in Scilit:

Age dependence of myocardial Na+-K+-ATPase activity and digitalis intoxication in the dog and guinea pig.
Circulation Research, 1981