Abstract
Comparisons of proteins synthesized by the sexually differentiated and non-differentiated cells of T. mesenterica IFO-9313 (mating type ab) were performed with the soluble cell-fraction prepared by centrifugation at 25,000 .times. g for 30 min. Two-dimensional gel electrophoresis demonstrated that alteration in synthesis of some cellular proteins is induced in response to a mating hormone, tremerogen A-10, secreted by T. mesenterica IFO-9310 (mating type AB). Typical example of alteration is as follows: cellular proteins a (MW, 30,000) and b (MW, 13,000), located by a region of an isoelectric point, around 5.8, are remarkably decreased and increased, respectively, during sexual differentiation. Gene control for sexual differentiation may occur in response to the extracellular information substance.

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