Patterns of filipin-sterol complex distribution in intact erythrocytes and intramembrane particle-aggregated ghost membranes.
Open Access
- 1 July 1982
- journal article
- abstracts
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 30 (7) , 702-706
- https://doi.org/10.1177/30.7.7108195
Abstract
When fixed erythrocytes are exposed to the cholesterol probe, filipin, and freeze-fractured, their membrane is labeled with only a limited number of typical, 25 nm diameter filipin-sterol complexes. In addition, the membrane contains many less distinct filipin-induced perturbations that give it an overall rippled appearance. In contrast, red cell ghosts subjected to conditions that result in an aggregation of their intramembrane particles have large particle-free membrane domains which are completely filled with 25 nm filipin-sterol complexes. These results suggest that some constraint exists in the intact erythrocyte membrane which restricts the formation of typical filipin-sterol complexes.Keywords
This publication has 0 references indexed in Scilit: