Affinity Chromatography of Phenylalanine Hydroxylase

1 April 1978

journal article
research article
Published by Wiley in European Journal of Biochemistry

Vol. 85 (2) , 357-363
https://doi.org/10.1111/j.1432-1033.1978.tb12247.x

Abstract

Four independent methods established that the structure of a previously reported pteridine affinity adsorbent, 6,7-dimethyl-5,6,7,8-tetrahydropterin.sbd.CH-Sepharose, is 5(CH-Sepharosyl)-6,7-dimethyl-5,6,7,8-tetrahydroprotein. A novel reaction, the carbodiimide-promoted coupling of a carboxyl group to N-5 of a tetrahydropterin, is described. Two novel adsorbents, 5-formyl-tetrahydrofolate.sbd.AH-Sepharose and 5-methyl-tetrahydrofolate.sbd.AH-Sepharose, are described which may be useful not only in the study of phenylalanine hydroxylase (EC 1.14.16.1) but also in the study of folate-metabolizing enzymes.

This publication has 17 references indexed in Scilit:

Dihydroxanthopterinuria in phenylketonuria and lethal hyperphenylalaninemia patients
Clinica Chimica Acta; International Journal of Clinical Chemistry, 1977
Purification of inactive phenylalanine hydroxylase protein from liver in classical phenylketonuria
Nature, 1976
Phenylalanine Hydroxylase of Macaca irus
European Journal of Biochemistry, 1975
A pteridine adsorbent for affinity chromatography
FEBS Letters, 1974
Adsorbents for affinity chromatography. Use of N-hydroxysuccinimide esters of agarose
Biochemistry, 1972
Folate antagonists covalently linked to carbohydrates: Synthesis, properties, and use in the purification of dihydrofolate reductases
Archives of Biochemistry and Biophysics, 1972
Isolation of the folate‐binding protein from cow's milk by the use of affinity chromatography
FEBS Letters, 1972
Affinity chromatography of dihydrofolate reductase
Biochemical Journal, 1971
Purification of dihydrofolic reductase from chicken liver by affinity chromatography
Biochemical and Biophysical Research Communications, 1971
2-Amino-4-hydroxy-6-methyl-7,8-dihydropteridine as a Model for Dihydrofolate^*
Biochemistry, 1967