Untersuchungen über das Protoplasma grüner Pflanzenzellen. IV. Die Lipoide der Spinatchloroplasten

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Abstract
Most of the ether-soluble material in spinach leaves is found in the chloroplasts. The chloroplast substance is obtained by extracting the leaves with H2O in a stream of CO2. The aqueous filtrate is frozen and dried in vacuo. The dried material is extracted with ether, then with ether-alcohol (1:3). The ether extract is dried in vacuo under N and weighed. The ether-alcohol extract is transferred to ether, the mixture filtered, and the ether filtrate treated the same as the ether extract. P (0.09-0.13% of the lipoids) is detd. in a small amt. of the dried extract, which is then dissolved in a little ether and treated with ice cold acetone. The acetone precipitate contains phosphatides and waxes (16-21% of the lipoids) while pigments, triglycerides and sterols remain in soln. (79-84% of the lipoids). The amt. of ether soluble P in the chloroplasts increases from spring to autumn. A small amt. of the new acetal phosphatide is present. The acetone-soluble fraction is saponified with KOH, the soaps decomposed with acetic acid and the fatty acids and chlorophyll decomposition products extracted with ether. The acetone-soluble fraction contains 45.33-55.25% fatty acids, 5.74-6.59% glycerol and 15.17-20.92% unsaponifiable material, of which 11.13-14.35% are sterols. The. neutralization nos. of the fatty acids are 196.3-203.5, the av. mol. wts., 275.7-285.5 and the I nos., 184.7-210.

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