Transformation by simian virus 40 induces virus-specific, related antigens in the surface membrane and nuclear envelope

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Abstract
Nucleus- and mitochondrion-free membranes from hamster lymphocytes transformed by SV-40, GD248 cells, cause guinea pigs to produce immune sera that reveal the presence in GD248 plasma membranes and mitochondria of 2 types of glycoprotein that are not detected in membranes of normal lymphocytes. Indirect immune fluorescence of living, SV-40-transformed T19 hamster reticulum cells, Balb/c 3T3 mouse fibroblasts and W18 VA2 human fibroblasts, using the antisera against GD248 membrane at 4.degree. C produced a distinct cell surface fluorescence; above 20.degree. staining at the nuclear perimeter, the SV-40 U-antigen reaction, becomes equally prominent. In SV-40-transformed cells that were fixed in cold acetone and in purified GD248 nuclei, thermostable U-antigen staining is dramatic, but there is no reaction for nuclear T[tumor]-antigen. Rabbit antisera against T19 cells gave immunofluorescence reactions equivalent to those obtained with the antisera against GD248 cells. Normal guinea pig or rabbit sera and cells that were not transformed by SV-40 gave no reaction. Sera from tumor-bearing hamsters gave only nuclear T-antigen fluorescence. The results indicate the presence of related, SV-40-specific antigens in the surface membranes, nuclear envelope and possibly other intracellular organelles of SV-40-transformed cells.
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