Isozyme Characterization of Sorghum Aphid Species and Greenbug Biotypes (Homoptera: Aphididae)

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Abstract
Seven isolates of greenbugs, Schizaphis graminum (Rondani), and one isolate each of the corn leaf aphid, Rhopalosiphum maidis (Fitch), and the yellow sugarcane aphid, Sipha flava (Forbes) were tested for diagnostic isozymes. Of 12 isozyme systems tested, six gave no reaction with aphid extracts. Anodal and cathodal esterase showed isozyme patterns for aphids and corn or sorghum extracts, but the banding patterns did not show any differences among aphid species. Malate dehydrogenase and phosphoglucomutase assays demonstrated isozyme differences among aphid species but not among greenbug isolates. Phosphohexose isomerase assay showed both inter- and intraspeciflc differences (greenbugs), but the intraspecific differences were not reproducible. One enzyme, 6-phosphogluconate dehydrogenase, differed interspecifically and intraspecifically. Three electromorphs of greenbugs and the single one of corn leaf aphid and one of yellow sugarcane aphid were identified by electrophoresis. The electromorph groupings of the greenbugs were consistent with biotypes identified by plant host studies and mitochondrial DNA analysis.

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