GROWTH OF HERPES SIMPLEX VIRUS IN TRANSFORMED GLIAL AND NEURONAL CELLS IN TISSUE CULTURE

Publisher Website
Google Scholar
Abstract
The production of herpes simplex virus by transformed neuronal and glial cells in continuous tissue culture was studied by plaque formation techniques and electron microscopy. It was found that i) transformed neuronal cells routinely produced 100–1000 fold more infectious virus particles than do similarly infected transformed glial cells, and ii) in the electron microscope, envelopment of nucleocapsids at the glial cell nuclear membrane is inefficient with few enveloped particles produced. In the glial cells, infection causes a loss of integrity of the nuclear membrane. The differential response of these neuronal and glial cells in continuous tissue culture to HSV infection may reflect different responses occurring in these cell types in vivo.

This publication has 0 references indexed in Scilit: