Three-Dimensional Image Analysis of the Complex of Thin Filaments and Myosin Molecules from Skeletal Muscle. III. The Multi-Domain Structure of Actin-Heavy Meromyosin Complex1
- 30 June 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 90 (3) , 703-714
- https://doi.org/10.1093/oxfordjournals.jbchem.a133524
Abstract
Heavy meromyosin (HMM) prepared by chymotryptic digestion retains DTNB light chain and subfragment-2 (S2) in addition to the two subfragment-1 (S1) moieties. The electron micrograph of negatively stained rigor complex of HMM and actin showed some different features as compared with that of S1 and actin. The three-dimensional image reconstructed from the electron micrograph of actin-HMM gave additional domains other than those observed in actin-Sl (Wakabayashi and Toyoshima, 1981). The differences between the images of actin-HMM and actin-S1 were attributed to the differences in protein composition. Further, the structural characteristics of actin-HMM are discussed in comparison with those of actin-Sl.Keywords
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