Isolation of a transposable element from Neurospora crassa.
- 1 March 1989
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 86 (6) , 1929-1933
- https://doi.org/10.1073/pnas.86.6.1929
Abstract
A Neurospora crassa strain from Adiopodoume, Ivory, Coast, contains multiple copies of a transposable element, Tad. The element was detected as a 7-kilobase insertion in two independently isolated spontaneous forward mutants of the am (glutmate dehydrogenase) gene. Laboratory strains do not contain Tad. All progeny from crosses of the Adipodoume strain to laboratory strains contain multiple copies. When the element was inserted in am, target sequences of 14 and 17 base pairs were duplicated in the two cases analyzed. One mutation, caused by the insertion of Tad at the beginning of the am coding sequence, is genetically stable. The other mutation, caused by insertion upstream of the transcriptional start site, has a reversion frequency of 2.5 .times. 10-3. Precise excisions of Tad have not been found.This publication has 27 references indexed in Scilit:
- An extrachromosomal plasmid is the etiological precursor of kalDNA insertion sequences in the mitochrondrial chromosome of senescent neurosporaCell, 1986
- MOLECULAR GENETICS OF TRANSPOSABLE ELEMENTS IN PLANTSAnnual Review of Genetics, 1986
- Mitochondrial plasmids of neurospora: Integration into mitochondrial DNA and evidence for reverse transcription in mitochondriaCell, 1986
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1984
- The complete nucleotide sequence of the Neurospora crassa am (NADP-specific glutamate dehydrogenase) geneGene, 1983
- The molecular organization of the Antennapedia locus of drosophilaCell, 1983
- The Notch locus of Drosophila melanogasterCell, 1983
- Regulation of white locus expression: The structure of mutant alleles at the white locus of Drosophila melanogasterCell, 1982
- DNA rearrangements associated with a transposable element in yeastCell, 1980
- A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymesGene, 1980