HtrA is a key factor in the response to specific stress conditions inLactococcus lactis
Open Access
- 1 July 2003
- journal article
- Published by Oxford University Press (OUP) in FEMS Microbiology Letters
- Vol. 224 (1) , 53-59
- https://doi.org/10.1016/s0378-1097(03)00419-1
Abstract
We investigated the physiological role of Lactococcus lactis housekeeping surface protease HtrA. It is involved in surface properties under regular growth conditions, as the htrA mutant strain forms longer chains in liquid medium. It participates in cellular defence against environmental stress conditions: compared to the wild-type strain, the htrA mutant strain exhibited increased sensitivity to heat, ethanol, puromycin, and NaCl, but not to pH, H2O2, bile salts or to carbon or nitrogen starvation. htrA transcription in the wild-type strain showed a transient increase under stress conditions determined as requiring htrA, but not under overexpression of a secreted heterologous protein. Our results demonstrate that in L. lactis, htrA is a key factor in the response to specific stress conditions.Keywords
This publication has 32 references indexed in Scilit:
- The HtrA Family of Proteases: Implications for Protein Composition and Cell FatePublished by Elsevier ,2002
- Role of the htrA Gene in Klebsiella pneumoniae VirulenceInfection and Immunity, 2002
- Crystal structure of DegP (HtrA) reveals a new protease-chaperone machineNature, 2002
- Conserved DegP Protease in Gram-Positive Bacteria Is Essential for Thermal and Oxidative Tolerance and Full Virulence in Streptococcus pyogenesInfection and Immunity, 2001
- YkdA and YvtA, HtrA-Like Serine Proteases in Bacillus subtilis , Engage in Negative Autoregulation and Reciprocal Cross-Regulation of ykdA and yvtA Gene ExpressionJournal of Bacteriology, 2001
- Environmental stress responses inLactococcus lactisFEMS Microbiology Reviews, 1999
- Characterization of degQ and degS, Escherichia coli genes encoding homologs of the DegP proteaseJournal of Bacteriology, 1996
- Two distinct loci affecting conversion to mucoidy in Pseudomonas aeruginosa in cystic fibrosis encode homologs of the serine protease HtrAJournal of Bacteriology, 1996
- The role of a stress‐response protein in Salmonella typhimurium virulenceMolecular Microbiology, 1991
- The HtrA (DegP) protein, essential for Escherichia coli survival at high temperatures, is an endopeptidaseJournal of Bacteriology, 1990