The Culturing of Fresh-Water Protozoa and Other Small Invertebrates
- 1 November 1935
- journal article
- research article
- Published by University of Chicago Press in The American Naturalist
- Vol. 69 (725) , 628-632
- https://doi.org/10.1086/280632
Abstract
Methods for the culture of Amoeba, Arcella, Actinosphaerium, Stylonichia, Oxytricha, Chilomonas, Paramoecium, Colpidium, Colpoda, Euplotes, Didinium, Euglena, Hydra, Stenostomum, Pianaria, Philodina, Cyclops, Daphnia, Naias, Aelosoma, and Dero are described. A general stock culture solution, diluted 1:10 with distilled water before use, consisting of NaCl 12 g., KC1 0.03 g., CaCl2 0.04 g., NaHCO3 0.2 g., Phosphate buffer solution of a pH 6.9-7, 50 cc, distilled water to 1000 cc. and added to a rice-agar base in finger bowls, is suggested for most of the protozoa. For ciliates, such as Paramoecium and Colpidium, and Euplotes, a medium of egg yolk in water is found useful.This publication has 0 references indexed in Scilit: