The influence of medium formulation on phosphomonoester and UDP‐Hexose levels in cultured human colon tumor cells as observed by 31P NMR spectroscopy

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Abstract
High‐resolution 31P NMR spectroscopy at 11.7 T was used to examine the influence of medium formulation (medium and serum type, and concentrations of glucose and inositol) on the cellular phosphate metabolism of CX‐1 cells, a human colon cancer cell line derived from HT‐29 cells. Striking differences in the 31P spectra of harvested CX‐1 cells were observed. The largest variation was seen in the phosphocholine and UDP‐hexose levels (up to seven‐fold changes), with smaller differences in the levels of other phosphate metabolites. The major UDP‐hexose species were found to be UDP‐N‐acetylglucosamine and UDP‐N‐acetylgalactosamine (ca 2:1 ratio), which have been proposed in the literature to be markers of cell differentiation status. Medium‐induced alterations in metabolite levels were much greater than the normal variations seen in CX‐1 control samples grown under identical conditions. They even exceeded the characteristic differences observed between different human tumor cell lines grown under one set of culture conditions. The remarkable sensitivity of CX‐1 cellular phosphate metabolism to the culture environment has implications for the comparison of in vitro vs in vivo spectra, and for the interpretation of effects due to growth and therapy.