Microwave irradiation and in vitro release of enzymes from hepatic lysosomes

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Abstract
An exposure system for examining in vitro effects of microwave irradiation on cellular and subcellar components has been developed. The system was used to test the effect of 2.45-GHz CW microwaves on the release of two lysosomal enzymes. At a specific absorption rate (SAR) of 10, 50 or 100 mW/g (90 min) no effects were noted at 37° C (pH of 7.3) on lysosomal fragility as determined by the release of the lysosomal enzymes cathepsin D andβ-glucuronidase. When the medium was adjusted to pH 5.0, microwave irradiation of the lysosomal suspension had no effect on the acid-induced enhancement of release of lysosomal enzymes. The data indicate that microwave irradiation had no labilizing effect on the lysosomal membrane, although other microwave-membrane interactions not associated with enzyme release may occur.