Kinetic assay of beta-hydroxybutyrate in plasma with a COBAS-BIO centrifugal analyzer.
Open Access
- 1 April 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 28 (4) , 702-703
- https://doi.org/10.1093/clinchem/28.4.702
Abstract
An enzymic rate method for measuring beta-hydroxybutyrate in plasma by use of beta-hydroxybutyrate dehydrogenase (EC 1.1.1.30) was adapted to the Roche COBAS-BIO centrifugal analyzer. Optimum reagent composition and reaction conditions were determined, to provide increased sensitivity and accuracy. Use of magnesium ions increased sensitivity. Comparison of results with those of a manual enzymic endpoint method involving perchloric acid protein-free filtrates of whole blood showed excellent correlation (r = 0.996). The method has good within-run and day-to-day precision. Linearity of the standard curve extends to 5000 mumol of beta-hydroxybutyrate per liter. The assay can be completed in less than 5 min and requires 15 microL of plasma and minimal reagent volumes.This publication has 2 references indexed in Scilit:
- Direct assays of lactate, pyruvate, beta-hydroxybutyrate, and acetoacetate with a centrifugal analyzer.Clinical Chemistry, 1978
- Enzymic determination of d(−)-β-hydroxybutyric acid and acetoacetic acid in bloodBiochemical Journal, 1962