High levels of a heat-labile calmodulin-binding protein (CaM-BP80) in bovine neostriatum
- 1 April 1980
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 19 (9) , 1831-1837
- https://doi.org/10.1021/bi00550a016
Abstract
Bovine brain contains a heat-labile, 80,000 dalton calmodulin-binding protein (CaM-BP80) which inhibits the calmodulin-dependent activities of cyclic 3'',5''-nucleotide phosphodiesterase, adenylate cyclase and Ca2+-ATPase in vitro. CaM-BP80 is composed of 2 polypeptides (60,000 and 18,500 daltons) present in a 1:1 ratio. An antibody directed against CaM-BP80 was raised in rabbits and a radioimmunoassay [RIA] was developed, having a sensitivity of 60 fmol of CaM-BP80. Using the RIA, the levels of CaM-BP80 in various bovine tissues were determined. The protein was found primarily in the brain, present in particularly high levels in the neostriatum. These results, together with immunohistochemical localization of CaM-BP80 at the postsynaptic densities and the microtubules of postsynaptic dendrites suggest that the protein may have a role in the cerebrum at the site of neurotransmitter action and at the level of microtubular function.This publication has 33 references indexed in Scilit:
- Partial purification of the Ca2+-Mg2+ ATPase activator from human erythrocytes: Its similarity to the activator of 3′:5′ — cyclic nucleotide phosphodiesteraseBiochemical and Biophysical Research Communications, 1977
- Phosphodiesterase protein activator mimics red blood cell cytoplasmic activator of (Ca2+-Mg2+)ATPaseBiochemical and Biophysical Research Communications, 1977
- Rat brain adenylate cyclaseArchives of Biochemistry and Biophysics, 1977
- Modulator binding protein. Bovine brain protein exhibiting the Ca2+-dependent association with the protein modulator of cyclic nucleotide phosphodiesterase.Journal of Biological Chemistry, 1977
- Conformational transition accompanying the binding of calcium(2+) ion to the protein activator of 3',5'-cyclic adenosine monophosphate phosphodiesteraseBiochemistry, 1977
- Isolation of coconut storage proteins by polyacrylamide-gel electrophoresisAnalytical Biochemistry, 1976
- A brain protein and its effect on the CA2+-and protein modulator-activated cyclic nucleotide phosphodiesteraseBiochemical and Biophysical Research Communications, 1976
- Regulation of adenylate cyclase from glial tumor cells by calcium and a calcium-binding protein.Journal of Biological Chemistry, 1976
- Cyclic 3':5'-nucleotide phosphodiesterase. Ca2+ confers more helical conformation to the protein activator.Journal of Biological Chemistry, 1976
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951