hosoba toge toge , a Syndrome Caused by a Large Chromosomal Deletion Associated with a T-DNA Insertion in Arabidopsis

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Abstract
We isolated a T-DNA-tagged mutant named hosoba toge toge ( hot ) in which a pleiotropic phenotype was observed in both the shoot and root throughout the life cycle. The phenotype and allelism indicated that the mutant has a defect in both the FASCIATA1 ( FAS1 ) gene and the FT gene located on the bottom arm of chromosome 1. Analysis of the junctions between the T-DNA ends and the plant genome suggested the presence of a 75.8-kbp deletion at the insertion site. In addition to FAS1 and FT , 13 genes were predicted to exist in the region corresponding to that deleted in hot . They include homologs of genes for type II inositol-1,4,5-triphosphate 5-phosphatase (IP5Pase), the β-chain of N -acetyl-β-glucosaminidase (NAGase), NADPH oxidoreductase of the ζ-crystallin family, polygalacturonase, and endo-1,4-β-glucanase. Although most aspects of the hot phenotype can be explained by loss of FAS1 and FT functions, some novel phenotypic features which may represent aspects of a mutant phenotype due to loss-of-function of other gene(s) were observed. One “wild-type” ecotype and a previously reported T-DNA insertion line, neither of which has any obvious phenotypic abnormality, carry a possible loss-of-function mutation in the ζ-crystallin homolog and in the NAGase β chain homolog, respectively.