Substitution of foreign protein sequences into a chimeric toxin composed of transforming growth factor alpha and Pseudomonas exotoxin.
Open Access
- 1 March 1991
- journal article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 11 (3) , 1751-1753
- https://doi.org/10.1128/mcb.11.3.1751
Abstract
TGF alpha-PE40 is a chimeric toxin made by replacing domain Ia of Pseudomonas exotoxin (PE) with transforming growth factor alpha (TGF alpha). We have now replaced a portion of domain Ib of PE with different polypeptides or an extra domain III of PE in transforming growth factor alpha-PE40 and maintained cell killing. Thus, TGF alpha-PE40 can be used to transport foreign protein sequences into the cytosol of cells.Keywords
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