Transcription patterns of amplified Dytiscus genes coding for ribosomal RNA after injection into Xenopus oocyte nuclei.

Publisher Website
Google Scholar
Abstract
Oocytes of the frog X. laevis were injected with purified circular DNA containing amplified rRNA genes of the water beetle D. marginalis. Nuclear contents of injected oocytes were spread and examined by EM. Most of the D. marginalis DNA seen in injected nuclei contained regions densely packed with polymerases and nascent transcripts. Apparently normal and abnormal patterns of transcription were observed. It may become possible to recognize the transcribed regions and immediate transcripts of cloned DNA molecules whose activity cannot be seem by EM of normal nuclei.