Stable integration of isolated cell membrane patches in a nanomachined aperture
- 21 August 2000
- journal article
- Published by AIP Publishing in Applied Physics Letters
- Vol. 77 (8) , 1218-1220
- https://doi.org/10.1063/1.1289490
Abstract
We present a method for integrating an isolated cell membrane patch into a semiconductor device. The semiconductor is nanostructured for probing native cell membranes for scanning probe microscopy in situ. Apertures were etched into suspended silicon-nitride layers on a silicon substrate using standard optical lithography as well as electron-beam lithography in combination with reactive ion etching. Apertures of 1 μm diam were routinely fabricated and a reduction in size down to 50 nm was achieved. The stable integration of cell membranes was verified by confocal fluorescence microscopy in situ.Keywords
This publication has 14 references indexed in Scilit:
- The Breakdown of Cell Membranes by Electrical and Mechanical StressBiophysical Journal, 1998
- Single molecule detection and underwater fluorescence imaging with cantilevered near-field fiber optic probesApplied Physics Letters, 1998
- A scanning force microscope for simultaneous force and patch-clamp measurements on living cell tissuesReview of Scientific Instruments, 1997
- Supported Membranes: Scientific and Practical ApplicationsScience, 1996
- Near-field optical microscopy in liquidsApplied Physics Letters, 1995
- A look at membrane patches with a scanning force microscopeBiophysical Journal, 1995
- Lipid-glass adhesion in giga-sealed patch-clamped membranesBiophysical Journal, 1994
- Elastic deformation and failure of lipid bilayer membranes containing cholesterolBiophysical Journal, 1990
- Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patchesPflügers Archiv - European Journal of Physiology, 1981
- Single-channel currents recorded from membrane of denervated frog muscle fibresNature, 1976