Destruction of Ethidium Bromide in Solution by Ozonolysis
- 1 January 1988
- journal article
- research article
- Published by Walter de Gruyter GmbH in Biological Chemistry Hoppe-Seyler
- Vol. 369 (2) , 1191-1194
- https://doi.org/10.1515/bchm3.1988.369.2.1191
Abstract
Ethidium bromide can be rapidly destroyed in aqueous solutions or in isoamyl alcohol by ozonolysis in the presence of H2O2 to give a mixture of organic acids. In a variety of buffers commonly used in recombinant DNA technology destruction of ethidium bromide was more than 99.9%. The yellow reaction mixture after ozonolysis was shown to be nonmutagenic. This method may be used in laboratories for the disposal of ethidium bromide wastes.This publication has 4 references indexed in Scilit:
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- Revised methods for the Salmonella mutagenicity testMutation Research/Environmental Mutagenesis and Related Subjects, 1983
- In vitro metabolic activation of ethidium bromide and other phenanthridinium compounds: Mutagenic activity in Salmonella typhimuriumMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1977
- Methods for detecting carcinogens and mutagens with the salmonella/mammalian-microsome mutagenicity testMutation Research/Environmental Mutagenesis and Related Subjects, 1975