Lipoxygenase Inhibition by Anadanthoflavone, a New Flavonoid from the Aerial Parts ofAnadenanthera colubrina

Abstract
Chemical investigation of the aerial parts of Anadenanthera colubrina led to the isolation of a new flavonoid named anadanthoflavone (1), along with 11 known compounds: alnusenol, lupenone, lupeol, betulinic acid, α-amyrin, β-amyrin, β-sitosterol, stigmasterol, apigenin, 4-hydroxybenzoic acid and cinnamic acid. The isolated compounds were evaluated for their inhibitory activity on human platelet 12-lipoxygenase (12-hLO), human reticulocyte 15-lipoxygenase (15-hLO) and soybean lipoxygenase-1 (15-sLO). Compound 1 was found to be active against 12-hLO and 15-hLO with IC50 values of 13 ± 3 μM and 17 ± 3 μM, respectively. Apigenin selectively inhibited the activity of 15-hLO (IC50 : 4.0 ± 1 μM), while lupenone, lupeol and α-amyrin were found active against 15-sLO (IC50 : 22 ± 3 μM, 35 ± 9 μM and 15 ± 3 μM, respectively).

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