Design and Applications of Rapid-Scan Spectrally Resolved Fluorescence Microscopy
- 1 December 2000
- journal article
- research article
- Published by SAGE Publications in Applied Spectroscopy
- Vol. 54 (12) , 1727-1733
- https://doi.org/10.1366/0003702001948989
Abstract
This paper addresses the design and applications of a spectrally resolved confocal fluorescence microscope that measures spectral information for each pixel in the image with a fast multi-point approach. The combined spatial and spectral resolution of the device is demonstrated by performing two-photon imaging of mixtures of fluorescently labeled microspheres. Overlapping emission spectra of dye solutions contained in microcapillaries are readily distinguished. Images of a human skin tissue section and a mouse kidney section are presented which demonstrate that the structure and spectra of biologic samples can be resolved.Keywords
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