Cloning an Aspergillus nidulans developmental gene by transformation.
Open Access
- 1 May 1985
- journal article
- research article
- Published by Springer Nature in The EMBO Journal
- Vol. 4 (5) , 1307-1311
- https://doi.org/10.1002/j.1460-2075.1985.tb03777.x
Abstract
We have developed a transformation system for Aspergillus nidulans giving a frequency of transformation high enough to screen a gene bank from which we were able to isolate and clone the A. nidulans developmental gene brlA by visual selection. The vector contains the selective marker argB+, and with it a frequency of transformation of 500 stable transformants/micrograms plasmid DNA can regularly be achieved. The evidence suggests that transformation is by integration but spontaneous excision of integrated plasmids is apparently frequent enough to allow the recovery of transforming plasmids in Escherichia coli.This publication has 18 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Transformation by integration in Aspergillus nidulansGene, 1983
- Cloning and characterization of the ornithine carbamoyltransferase gene from Aspergillus nidulansGene, 1983
- Transformation of Aspergillus nidulans by the orotidine-5′-phosphate decarboxylase gene of Neurospora crassaBiochemical and Biophysical Research Communications, 1983
- The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primersGene, 1982
- Vectors for Cloning in YeastPublished by Springer Nature ,1982
- Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequencesNature, 1981
- Transformation of yeast by a replicating hybrid plasmidNature, 1978
- ColE1 plasmid mobility and relaxation complexNature, 1978
- The use ofp-fluorophenylalanine with ‘master strains’ ofAspergillus nidulansfor assigning genes to linkage groupsGenetics Research, 1965