Marking of sited cells after electrophysiologic study.

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Abstract
Acinar and ductular cells of the submaxillary gland of the Syrian hamster were identified with respect to their resting potential. The technical innovation is a convenient method for identifying the precise cell source of any given electrophysiological measurements with an iontophoretic marking system; 2% FeCl3 in a 3 [image] KCl solution was employed in a microelectrode for obser3ving the electrophysiological response of a cell. For marking, immediately after observation of potentials, an external current is imposed and Fe+++ ions iontophore-tically deposited in the cell during a 20-sec interval. The tissue is then quick frozen, serial sectioned, Gomori-stained for iron, and counter-stained for cellular outline. Subsequent histologic examination makes correlation of responses observed with particular cells conclusive.

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