INVIVO AND INVITRO STUDIES OF EXPERIMENTAL OVARIAN ADENOCARCINOMA IN RATS

  • 1 January 1979
    • journal article
    • research article
    • Vol. 39  (3) , 1108-1112
Abstract
When 7,12-dimethylbenz[a]anthracene-impregnated sutures were directly applied to the ovarian parenchyma of 8 wk old Sprague-Dawley rats (the clipping method), adenocarcinomas developed in 29 (39%) of the 75 rats during the 50 wk observation period. When 20-methylcholanthrene was used, adenocarcinomas developed only in 1 (3%) of the 31 rats. The clipping method using 7,12-dimethylbenz[a]anthracene is satisfactory as an animal model of ovarian adenocarcinoma, which comprises 85-90% of human malignant ovarian tumors. Attempts were made to isolate cloned cell lines from these experimental ovarian adenocarcinomas in vitro, and 2 cloned cell lines were obtained. They were epithelioid and produced undifferentiated adenocarcinomas by back-transplantation into isologous newborn rats.