Drosophila mitochondrial transcription factor A (d-TFAM) is dispensable for the transcription of mitochondrial DNA in Kc167 cells
- 1 March 2001
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 354 (2) , 243-8
- https://doi.org/10.1042/0264-6021:3540243
Abstract
We have cloned cDNA encoding Drosophila mitochondrial (mt) transcription factor A (d-TFAM). RNA interference (RNAi) of d-TFAM by lipofection of haemocyte-derived Kc167 cells with double-stranded RNA reduced d-TFAM to less than 5% of the normal level. Reflecting the ability of TFAM to stabilize mtDNA, RNAi of d-TFAM reduced mtDNA to 40%. Nonetheless, transcription of the ND2 and ND5 genes and their mRNAs remained unchanged for 8 days of the duration of RNAi. We thus show that d-TFAM is not essential for the transcription of Drosophila mtDNA.Keywords
This publication has 22 references indexed in Scilit:
- Disulfide‐bonding between Drosophila laminin β and γ chains is essential for α chain to form αβγ trimerFEBS Letters, 1997
- A single mouse gene encodes the mitochondrial transcription factor A and a testis–specific nuclear HMG-box proteinNature Genetics, 1996
- Interaction of mtTFB and mtRNA Polymerase at Core Promoters for Transcription of Xenopus laevis mtDNAJournal of Biological Chemistry, 1996
- Distinct Roles for Two Purified Factors in Transcription of Xenopus Mitochondrial DNAMolecular and Cellular Biology, 1995
- In organello footprint analysis of human mitochondrial DNA: human mitochondrial transcription factor A interactions at the origin of replication.Molecular and Cellular Biology, 1994
- Mitochondrial transcription initiation. Variation and conservationJournal of Biological Chemistry, 1993
- A human mitochondrial transcriptional activator can functionally replace a yeast mitochondrial HMG-box protein both in vivo and in vitro.Molecular and Cellular Biology, 1993
- DNA binding properties of an HMG1-related protein from yeast mitochondria.Journal of Biological Chemistry, 1992
- The yeast mitochondrial RNA polymerase specificity factor, MTF1, is similar to bacterial sigma factors.Journal of Biological Chemistry, 1991
- A close relative of the nuclear, chromosomal high-mobility group protein HMG1 in yeast mitochondria.Proceedings of the National Academy of Sciences, 1991