CD of the synthetic RNA duplexes poly[r(A‐T)] and poly[r(A‐U)] in salt and ethanolic solutions

Publisher Website
Google Scholar
Abstract
Synthetic RNA poly[r(A‐T)] has been synthesized and its CD spectral properties compared to those of poly[r(A‐U)], poly[d(A‐T)], and poly[d(A‐U)] in various salt and ethanolic solutions. The CD spectra of poly[r(A‐T)] in an aqueous buffer and of poly[d(A‐T)] in 70.8% v/v ethanol are very similar, suggesting that they both adopt the same A conformation. On the other hand, the CD spectra of poly[r(A‐T)] and of poly[r(A‐U)] differ in aqueous, and even more so in ethanolic, solutions. We have recently observed a two‐state salt‐induced isomerization of poly[r(A‐U)] into chiral condensates, perhaps of Z‐RNA [M. Vorlíčková, J. Kypr, and T. M. Jovin, (1988) Biopolymers27, 351–354]. It is shown here that poly[r(A‐T)] does not undergo this isomerization. Both the changes in secondary structure and tendency to aggregation are different for poly[r(A‐T)] and poly[r(A‐U)] in aqueous salt solutions. In most cases, the CD spectrum of poly[r(A‐U)] shows little modification of its CD spectrum unless the polymer denatures or aggregates, whereas poly[r(A‐T)] displays noncooperative alterations in its CD spectrum and a reduced tendency to aggregation. At high NaCl concentrations, poly[r(A‐T)] and poly[r(A‐U)] condense into ψ (‐) and ψ (+) structures, respectively, indicating that the type of aggregation is dictated by the polynucleotide chemical structure and the corresponding differences in conformational properties.