MAPPING OF LARGE AREAS WITH THE ELECTRON MICROSCOPE

Open Access
Publisher Website
Google Scholar
Abstract
Carbon-stabilized formvar films are strong enough to support entire sections over large areas of a copper grid from which some of the grid squares have been cut out. This technique has been utilized to produce electron micrograph montages of the following nerves: the circumesophageal connective, the chela nerve, and the ventral cord of the crayfish, the circumesophageal connective of the roach, and the optic nerve of the mouse. Direct counts of the nerve fibers in the entire cross-sectional area of these nerves by including the submicroscopic ones, provides for the first time an accurate method for determining total numbers of nerve fibers.

This publication has 3 references indexed in Scilit: