AN UNUSUAL MORPHOLOGIC VARIANT OF BF-S

  • 1 January 1984
    • journal article
    • research article
    • Vol. 36  (2) , 346-351
Abstract
In the course of family studies of haplotypes of the alleles of the 6th chromosome loci HLA-A, C, B, D/DR, BF, C2, C4A, C4B and glyoxalase I, an unusual BF [properdin factor B] variant was encountered. Its mobility was similar to BF F but it appeared to have a lesser intensity after staining with Coomassie Blue, and it was demonstrated by crossed immunoelectrophoresis to be present in lower concentration. It was therefore designated BF FQL. This variant was found on the haplotype HLA-A1, B17, DR7, BF*FQL, C2*C, C4A*6, C4B*1, GLO2. All other haplotypes of this type so far identified carry the BF variant BF S. Following activation of serum samples with zymosan, BF was analyzed by both agarose electrophoresis and isoelectric focusing and immunofixation. On both treatments, serum with BF SFQL produced a Ba pattern identical to that of a sample which was BF S. The Bp pattern for F and S are similar but differ from those of the rare variants BF F1 and BF S1. The BP pattern of BF FQL was, as expected, the same as BF F or BF S. The variant is a mutant from BF S with mobility similar to BF F. The mutation seemed also to have resulted in a lower concentration of product than normal.