DETECTION OF LUXOL-FAST-BLUE POSITIVE CELLS IN HUMAN PROMYELOCYTIC LEUKEMIA-CELL LINE HL-60
- 1 January 1981
- journal article
- research article
- Vol. 9 (9) , 887-892
Abstract
Differentiation of HL-60 cells toward the eosinophilic series was not reported previously. Eosinophil granule specific staining with Luxol fast blue was used to determine if HL-60 cells could differentiate into the eosinophilic lineage. The specificity of the Luxol fast blue stain for cells of the eosinophilic series was substantiated by comparison of the staining of cells from a patient with an eosinophilic syndrome by Wright-Giemsa and Luxol fast blue positivity was most notable in cells found in colonies formed from HL-60 colongenic cells in semisolid agar medium. Colony and cluster formation was spontaneous but in the presence of medium conditioned by either human placental cells or the human monocyte-like cell line, GCT, Luxol fast blue positive colonies and clusters were detected at a higher frequency.This publication has 1 reference indexed in Scilit:
- Terminal differentiation of human promyelocytic leukemia cells induced by dimethyl sulfoxide and other polar compounds.Proceedings of the National Academy of Sciences, 1978