BIOLOGICAL AND IMMUNOLOGICAL PROPERTIES OF HUMAN PITUITARY FOLLICLE STIMULATING HORMONE OBTAINED BY STARCH GEL ELECTROPHORESIS

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Abstract
SUMMARY: A highly potent preparation of human follicle stimulating hormone (FSH) was submitted to electrophoresis in starch gel. A fraction containing luteinizing hormone activity was separated from the main fraction of FSH. The latter showed no luteinizing activity by the ovarian ascorbic acid depletion method with 125 times the minimum effective dose in the assay for FSH. Synergistic joint action (see p. 544) was shown between the two fractions. An antiserum raised to the follicle stimulating component was investigated by red cell haemagglutination-inhibition tests. Its titre was only slightly reduced after absorption with chorionic gonadotrophin and haemagglutination was inhibited by preparations of FSH containing 0·5 μg./ml. (1·5 mg. HMG 24/ml.), but not by solutions of chorionic gonadotrophin containing up to 100 i.u./ml. This is regarded as evidence that the antiserum is fairly specific for FSH.