Delayed activation of phospholipase D by gonadotropin‐releasing hormone in a clonal pituitary gonadotrope cell line (αT3‐1)

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Abstract
Stimulation of cultured pituitary cells from a gonadotrope lineage (αT3‐1) by the gonadotropin‐releasing hormone agonist analog [D‐Trp6]GnRH (GnRH‐A) resulted in a manifold increase in accumulation of phosphatidylethanol, a specific product of phospholipase D phosphatidyl transferase activity when ethanol is the phosphatidyl group acceptor. Levels of the natural lipid product of phospholipase D, phosphatidic acid, were increased 2–3‐fold. Activation of phospholipase D by GnRH‐A was dose‐ and time‐dependent and was blocked by a GnRH receptor antagonist [D‐pClPhe2,D‐Trp3.6]GnRH. GnRH‐A stimulated phospholipase D activity after a lag of 1–2 min. We conclude that in αT3‐1 gonadotropes GnRH receptor occupancy results in delayed activation of phospholipase D which could participate in late phases of gonadotrope regulation by the neurohormone.

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