DLC‐1, a Rho GTPase‐activating protein with tumor suppressor function, is essential for embryonic development
- 19 January 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (5) , 1191-1196
- https://doi.org/10.1016/j.febslet.2004.12.090
Abstract
DLC-1 (deleted in liver cancer 1) is a Rho GTPase-activating protein that is able to inhibit cell growth and suppress tumorigenesis. We have used homologous recombination to inactivate the mouse DLC-1 gene (Arhgap7). Mice heterozygous for the targeted allele were phenotypically normal, but homozygous mutant embryos did not survive beyond 10.5 days post coitum. Histological analysis revealed that DLC-1-/- embryos had defects in the neural tube, brain, heart, and placenta. Cultured fibroblasts from DLC-1-deficient embryos displayed alterations in the organization of actin filaments and focal adhesions.Keywords
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