Characterization of Na+/H+‐antiporter gene closely related to the salt‐tolerance of yeast Zygosaccharomyces rouxii

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Abstract
In order to clarify the relationship between salt‐tolerance of Zygosaccharomyces rouxii and the function of Na+/H+‐antiporter, a gene was isolated from Z. rouxii which exhibited homology to the Na+/H+‐antiporter gene (sod2) from Schizosaccharomyces pombe. This newly isolated gene (Z‐SOD2) encoded a product of 791 amino acids, which was larger than the product encoded by its Sz. pombe homologue. The predicted amino‐acid sequence of Z‐Sod2p was highly homologous to that of the Sz. pombe protein, but included an extra‐hydrophilic stretch in the C‐terminal region. The expression of Z‐SOD2 was constitutive and independent of NaCl‐shock. Z‐SOD2‐disruptants of Z. rouxii did not grow in media supplemented with 3 M‐NaCl, but grew well in the presence of 50% sorbitol, indicating that the function of Z‐SOD2 was closely related to the salt‐tolerance of Z. rouxii. Several genes are also compared and discussed in relation to the salt‐tolerance of Z. rouxii. The nucleotide sequence data reported in this paper will appear in the GSDB, DDBJ, EMBL and NCBI nucleotide sequence databases with the following accession number: D43629.