Radioimmunoassay of neurotensin and the distribution and concentration of gut neurotensin in rat and dog.
- 1 January 1980
- journal article
- research article
- Published by Tohoku University Medical Press in The Tohoku Journal of Experimental Medicine
- Vol. 130 (2) , 129-137
- https://doi.org/10.1620/tjem.130.129
Abstract
Four antisera of high avidity were raised in 4 rabbits immunized with synthetic NT [neurotensin] tridecapeptide coupled with bovine serum albumin via glutaraldehyde. NT was labeled with Na125I by means of modified Hunter-Greenwood''s chloramine-T method. Sensitivity of the RIA [radioimmunoassay] was 100 pg/ml. Cross-reactivity of antiserum to other peptides was not observed. Using the RIA, the distribution and concentration of gastrointestinal (GI)-NT were determined in acid-acetone extracts of rat and dog GI tissues. RIA using 4 kinds of antisera similarly detected higher concentrations in jejuno-ileal sections than in other regions. Although the distribution of GI-NT was quite similar between rat and dog, its concentration showed species specific difference.This publication has 2 references indexed in Scilit:
- Cardiovascular and Metabolic Actions of Neurotensin and (Gln4)‐NeurotensinActa Physiologica Scandinavica, 1976
- Radioimmunoassay for neurotensin, a hypothalamic peptide.Journal of Biological Chemistry, 1976