Expression, function and regulation of the intercellular adhesion molecule‐1 (ICAM‐1) on human intestinal epithelial cell lines

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Abstract
We have characterized the presence of the intercellular adhesion molecule‐1 (ICAM‐1) (CD54) on human intestinal adenocarcinoma cell lines as a nonreducible polypeptide of Mr 93 kDa, identified as a rhinovirus receptor. Expression of ICAM‐1 was positively correlated with enterocytic maturation, in that the percentage of ICAM‐1+ cells was highest in the most differentiated cell line Caco‐2. ICAM‐1 could be up‐regulated only on the less differentiated cell lines HT29 and T84 by phorbol 12‐myristate 13‐acetate and by the cytokines interferon‐gamma (IFN‐γ) and interleukin (IL) 1β. Enterocyte ICAM‐1 was involved in adhesion to activated T cells through binding to the leukocyte function associated antigen‐1 (LFA‐1). These data provide evidence that colon adenocarcinoma cell lines express functional ICAM‐1 sensitive to cytokine regulation. These findings support the hypothesis that lympho‐epithelial interactions involving the ICAM‐1/LFA‐1 pathway may be implicated in immunosurveillance of colon adenocarcinomas, inflammatory bowel disease and celiac disease, where increased levels of proinflammatory cytokines are locally produced within the gut mucosa.

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