Purification of Human Leukocytes.

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Abstract
Methods for preparing human leukocytes from venous blood were investigated. Sedimentation of erythrocytes by fibrinogen or phytohemagglutinin of Phaseolus vulgar is gave the best results. With white cell purity as a goal, the phytohemagglutinin method has been superior, giving preparations which were 72.7% and 96.5% WBC from normal and leukemic bloods respectively. These values have been increased to 93.8% and 98.8% by utilization of a phosphate buffer that preferentially lyses red cells while leaving white cells intact.
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