FLIP-FLOP IN THE LYT-2 PHENOTYPE OF T-CELLS FROM RADIATION CHIMERAS BETWEEN THY-1 CONGENIC DONOR AND RECIPIENT MICE

  • 1 January 1984
    • journal article
    • research article
    • Vol. 51  (3) , 563-570
Abstract
Evidence is presented that T helper cells change their surface phenotype from Lyt 2- to Lyt 2+, after secondary adoptive transfer. Chimaeras were established with Lyt 2- [mouse] spleen cells from normal or carrier-primed A-Thy 1a donors and irradiated A-Thy 1b recipients. Despite efficient depletion of Lyt 2+ cells from the initial donor, in the fluorescence-activated cell sorter, the majority of chimaeric T cells expressed Lyt 2 and were resistant to monoclonal Thy 1.2 alloantibody and complement treatment. In functional studies, the T helper activity of chimaeras reconstituted with carrier-primed spleen cells, was present in both Lyt 2+ and Lyt 2- subsets.

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