A simple method for the quantitative isolation and resolution of unesterified fatty acids from fats and oils

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Abstract
The unesterified fatty acids (UFA) present in fats and oils are isolated cleanly and without the formation of detectable artifacts due to saponification. The lipid (≤0.5 g) dissolved in 5 ml of n‐hexane is passed over a 300‐mg bed of Celite impregnated with saturated, aqueous Na3PO4 so that the solution passes through the bed in 3–4 min. After the bed has been washed, the UFA are freed from their salts by pumping of HC1 vapor over the bed. The acids are eluted with CH2Cl2 and subsequently separated underivatized by gas liquid chromatography. The C10 through C18.3 acids are separated in 10‐C18∶0 and C18∶1, C18∶2 and C18∶3 acids added to fatty acid‐free fats and oils in several concentrations was nearly 100%