Probing eukaryotic RNA polymerases B with monoclonal antibodies
- 25 July 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 158 (2) , 343-348
- https://doi.org/10.1016/0014-5793(83)80610-3
Abstract
Monoclonal antibodies directed against RNA polymerase B of the fungus Podospora comata were selected on the basis of different subunits recognition and inhibitory effect on enzyme activity. A library of 10 antibodies biased toward B180, B145, B39, B23,5 and B11 subunits was constructed. Most of these anitbodies also recognize yeast, wheat germ and calf thymus RNA polymerase B. Subunits bearing antigenic determinants are not always homologous in Podospora and yeast enzyme. As some of these antibodies strongly inhibit enzyme activity they constitute potent probes for functional studies of corresponding subunitsKeywords
This publication has 3 references indexed in Scilit:
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- Monoclonal antibody directed against RNA polymerase II of Drosophila melanogasterMolecular Genetics and Genomics, 1980
- Production of monoclonal antibodies: Strategy and tacticsJournal of Immunological Methods, 1980